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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
11/12/2019 |
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Data da última atualização: |
11/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ÁVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. |
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Afiliação: |
Lucas J. L. Pizauro, UNESP; Camila C. de Almeida, UNESP; Glenn A. Soltes, University of Guelph; Durda Slavic, University of Guelph; Fernando A. de Ávila, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Janet I. MacInnes, University of Guelph. |
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Título: |
Detection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Dairy Science, v.102, n.12, dec. 2019. |
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Páginas: |
p.11459-11464 |
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DOI: |
https://doi.org/10.3168/jds.2018-15920 |
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Idioma: |
Inglês |
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Notas: |
Short communication. |
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Conteúdo: |
The aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes. MenosThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also dete... Mostrar Tudo |
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Palavras-Chave: |
Dairy buffalo; Virulence genes. |
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Thesaurus Nal: |
Antibiotic resistance; Biofilm. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02937naa a2200277 a 4500
001 2116604
005 2019-12-11
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2018-15920$2DOI
100 1 $aPIZAURO, L. J. L.
245 $aDetection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment.$h[electronic resource]
260 $c2019
300 $ap.11459-11464
500 $aShort communication.
520 $aThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes.
650 $aAntibiotic resistance
650 $aBiofilm
653 $aDairy buffalo
653 $aVirulence genes
700 1 $aALMEIDA, C. C. de
700 1 $aSOLTES, G. A.
700 1 $aSLAVIC, D.
700 1 $aÁVILA, F. A. de
700 1 $aZAFALON, L. F.
700 1 $aMACINNES, J. I.
773 $tJournal of Dairy Science$gv.102, n.12, dec. 2019.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca |
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| Registros recuperados : 8 | |
| 1. |  | PIZAURO, L. J. L.; ALMEIDA, C. C. de; ROSSI JÚNIOR, O. D.; ÁVILA, F. A. de; ZAFALON, L. F. Isolamento de Staphylococcus coagulase-negativos e variação na contagem de células somáticas em amostras de leite bubalino. Revista Brasileira de Higiene e Sanidade Animal, v. 8, n. 5, (Supl. 1), p. 295-305, 2014.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|    |
| 2. | | PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ROSSI JUNIOR, O. D.; AVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR. Veterinary Microbiology, v. 204, p. 8-14, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 3. | | PIZAURO, L. J. L.; ALMEIDA, C. C. de; GOHARI, I. M.; MACINNES, J. I.; ZAFALON, L. F.; KROPINSKI, A. M.; VARANI, A. M. Complete genome sequences of 11 Staphylococcus sp. Strains Isolated from buffalo milk and milkers? hands. Microbiology Resource Announcements, v. 8, n. 47, e01264-19, nov. 2019. 3 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 4. | | PIZAURO, L. J. L.; ALMEIDA, C. C. DE; SILVA, S. R.; MACINNES, J. I.; KROPINSKI, A. M.; ZAFALON, L. F.; AVILA, F. A. DE; VARANI, A. DE M. Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. Scientific Reports, v.11, 2021, 17392. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 5. | | PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ÁVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. Detection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment. Journal of Dairy Science, v.102, n.12, dec. 2019. p.11459-11464 Short communication.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 6. | | CASTELANI, L.; PIZAURO, L. J. L.; MUSSI, F. L.; MOTTA, T. P.; POZZI, C. R.; ARCARO, J. R. P.; ZAFALON, L. F. Perfil de resistência antimicrobiana e produção de biofilme por Staphylococcus aureus isolados de novilhas. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26, 2011, Foz do Iguaçu, 2011. Anais... Foz do Iguaçu: SBM, 2011| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | ALMEIDA, C. C. de; PIZAURO, L. J. L.; ROSSI JÚNIOR, O. D.; ÁVILA, D. A. de; ZAFALON, L. F.; PIZAURO, J. M. Isolamento e identificação de Staphylococcus aureus resistentes à antimicrobianos em amostras de leite bubalino. Revista Brasileira de Higiene e Sanidade Animal, v. 8, n. 5, Supl. 1, p. 366-378, set. 2014.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 8. | | VALMORBIDA, M. K.; CARDOZO, M. V.; ALMEIDA, C. C. DE; PEREIRA, N.; DEZEN, D.; ASSIS, M. Z. DE; VERBISCK, N. V.; GRIEBELER, E.; PIZAURO, L. J. L.; ÁVILA, F. A. DE. Association between coa gene and enterotoxin gene in S. aureus from dairy cattle in Brazil. Food Science and Technology, 43, e16222, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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